Purification of antifungal pepitde from Aspergillus fumigatus / 中国生化药物杂志

ABSTRACT

Purpose　The aim is to purify the peptide with antifu ngal activity from Aspergillus fumigatus culture fluid.Methods　 The pept ide was separated by ion exchange column chromatography and further purified by r everse phase HPLC,and the molecular weight was determined by tricine gel electro phoresis.Results　A kind of peptide(FIP) with antifungal activit y wa s separated. The molecular weight was about 8 000. The detection result by MTT method showed significant antifungal activity of the peptide to 5 kinds of fung i.Conclusion　 The study provided a reliable basis for developin g drug and antiseptic.