Assays for detection of human papillomavirus infection in diagnosis of high grade cervical lesions / 中华临床感染病杂志
Chinese Journal of Clinical Infectious Diseases
; (6): 83-86, 2011.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-413846
Responsible library:
WPRO
ABSTRACT
Objective To evaluate the application of different assays for detection of human papillomavirus(HPV)in diagnosis of high grade cervical lesions.Methods Two hundred subjects with abnormal thinprep liquid-based cytology test(TCT)Resultswere selected for HPV DNA detection by hybrid capture 2(HC-Ⅱ) and Methodsbased on PCR including flow-through hybridization and gene chip (HybriMax),real-time fluorescent quantitative PCR(FQ-PCR)and flow fluorescent hybridization assay.Cytopathological Resultswere used as gold standards to evaluate the test performance of the above assays for diagnosing cervical intraepithelial neoplasia(CIN)≥Ⅱ. SPSS 13.0 software was used for statistical analysis.Results HPV DNA positive rates of 200 samples by HybriMax,FQ-PCR,flow fluorescent hybridization assay and HC-Ⅱ were 72.5%(145/200),71.5%(143/200),70.0%(140/200)and 69.0%(138/200),respectively,and the differences were not statistically si(g)nificant(x2 =0.252,0134,0.012 and 0.027,P > 0.05).The sensitivity,Youden index and negative predictive value of the above assays were statistically different(x2 =7.923,7.819 and 8.108,P <0.05).Conclusion HC-Ⅱ is superior to PCR Methodsin diagnosis of CIN Ⅱ and above.
Full text:
Available
Database:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
/
Practice guideline
Language:
Chinese
Journal:
Chinese Journal of Clinical Infectious Diseases
Year:
2011
Document type:
Article