Effect of Shenxiong-Huayu capsule preconditioning on cell apoptosis and the expression of C-fos and C-jun in the hippocampal CA1 area of rats with cerebral ischemia reperfusion injury / 国际中医中药杂志

ABSTRACT

ObjectiveTo observe the effect of Shenxiong-Huayu Capsule preconditioning on cell apoptosis and the expression of c-fos、c-jun in the hippocampal CA1 area of rats with acute cerebral ischemia reperfusion injury.Methods SD rats were divided into 3 groups by completely randomized method:sham operation group(n=6),ischemia reperfusion group (model group),and Shenxiong-Huayu Capsule preconditioning group (preconditioning group).The last two groups were divided randomly into 6 h,24 h,48 h and 72 h reperfusion subgroups (each n=6).Intragastric administration for 7 days,once a day.Middle cerebral artery occlusion and reperfushion model was made by an intraluminal filament method.Cell apoptosis was detected by TUNEL method,the expression of c-fos、c-jun was observed by immunohistochemistry.Results ① The number of apoptosic cells at 6、24、48、72hin the hippocampal CA1 area ofrats was respectively (11.17±3.71、39.83 ± 5.67、48.33± 5.32 and 22.17± 3.7 1) single/High power field in the model group,and was respectively (7.83±2.04、15.00±3.58、29.50±6.89 and 10.17±2.32) single/High power field in Shenxiong-Huayu Capsule preconditioning group.Compared with the model group,the number of apoptosic cells at each time point in the hippocampal CA1 area of rats was decreased in Shenxiong-Huayu Capsule preconditioning group(P＜0.05 or P＜0.01 ).② The number ofc-fos at 6、24、48、72 h in the hippocampal CA1 area of rats was respectively (14.50± 3.45、33.67± 1.63、42.33±3.32 and 32.00 ±2.90) single/High power field in the model group,and was respectively (10.17 ± 2.93、21.50 ± 2.43、30.83 ± 3.76 and 25.17 ± 5.27) single/High power field in Shenxiong-Huayu Capsule preconditioning group.The number of c-jun at 6、24、48、72 h in the hippocampal CA1 area of rats was respectively (15.50±4.19、22.83±5.64、33.10±4.19 and 14.67±3.08) single/High power field in the model group,and was respectively (9.67± 3.63、15.67±2.73、21.26±3.63 and 9.33 ±3.61)single/High power field in Shenxiong-Huayu Capsule preconditioning group.Compared with the model group,the expression of c-fos、c-jun at each time point in the hippocampal CA1 area of rats was decreased in Shenxiong-Huayu Capsule preconditioning group (P＜0.05 or P＜0.01).ConclusionShenxiong-Huayu capsule can inhibit apoptosis by restraining the expression of c-fos、c-jun and relive cerebral ischemia reperfusion injury in rats.