Effect of naloxone on L-type calcium current in isolated rat ventricular myocytes / 中华麻醉学杂志
Chinese Journal of Anesthesiology
; (12): 926-928, 2011.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-422419
Responsible library:
WPRO
ABSTRACT
ObjectiveTo evaluate the effect of naloxone on L-type calcium current (ICa-L) in isolated rat ventricular myocytes.MethodsAdult SD rats of both sexes aged 8 weeks weighing 200-250 g were used in this study.Single cardiac ventricular myocytes were enzymatically isolated from SD rats.ICa-L was measured in ventricular myocytes and recorded using whole cell patch-clamp technique.Different concentrations (20 and 100 μg/ml) of naloxone were added to the cardiomyocytes.The effect of naloxone on ICa-L was evaluated.ResultsThe peak current of ICa-L Was inhibited by naloxone in a concentration-dependent manner.Naloxone had no significant effect on steady-state activation curve.ConclusionNaloxone inhibits the L-type calcium channel of ventricular myocytes and exerts negative effect on ventricular muscle function.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Anesthesiology
Year:
2011
Document type:
Article