Performance of HCV and HIV-1 Nucleic Acid Amplification Test(NAT) in Korean Blood Donors / 대한수혈학회지
Korean Journal of Blood Transfusion
; : 91-97, 2000.
Article
in Ko
| WPRIM
| ID: wpr-42776
Responsible library:
WPRO
ABSTRACT
BACKGROUND: There is still risk of acquiring HCV and HIV by transfusion due to window phase. Screening for HCV and HIV-1 by nucleic acid amplification testing (NAT) may improve blood safety allowing detection during the preseroconversion window in donors. METHODS: We investigated NAT usefulness using COBAS AMPLICOR analyzer (Roche). The following sample population were tested:1) 15,552 HCV/HIV-1 seronegative random blood donor samples for HCV and HIV-1 NAT;2) 696 high ALT and 271 HCV EIA positive samples for HCV NAT;3) 1,152 HIV-1 EIA reactive samples for HIV-1 NAT. NAT was performed on pools of 24 donations according to the assay protocol. RESLUTS: Six pools showed initial reactive reactions in HCV NAT and one pool showed initial reactive reaction in HIV-1 NAT. But no donor sample was found repeatedly reactive by this assay. CONCLUSIONS: Although there were false positive reactions, specificity of the NAT assay was high enough for the assay to be applied as a blood screening test and implementation of this assay is expected to improve blood safety and be useful for blood products use.
Key words
Full text:
1
Database:
WPRIM
Main subject:
Tissue Donors
/
Blood Donors
/
Mass Screening
/
Sensitivity and Specificity
/
HIV-1
/
HIV
/
Nucleic Acid Amplification Techniques
/
False Positive Reactions
/
Blood Safety
Type of study:
Diagnostic_studies
/
Screening_studies
Limits:
Humans
Language:
Ko
Journal:
Korean Journal of Blood Transfusion
Year:
2000
Document type:
Article