HPLC fingerprint analysis on Shuangbai Powder / 中成药

ABSTRACT

AIM:To establish the method of fingerprint analysis on Shuangbai Powder(Radix et Rhizoma rhei,Cacumen platycladi,Cortex phellodendri amurensis,Herba lycopi and Herba menthae)to distinguish the characteristic fingerprint.METHODS:HPLC with ZORBAX Eclipse XDB-C_(18) was used,acetonitrile-0.1% H_3PO_4 solution(gradient elution)as a mobile phase and detection wavelength at 254 nm,flow rate was 1 mL/min,and column temperature was 40℃.RESULTS:Thirty-seven common peaks were separated from 10 batches of Shuangbai Powder.The characteristic peaks were the summation,22 peaks were from Radix et Rhizoma Rhei,6 peaks were from Cortex phellodendri Amurensis,4 peaks were from Cacumen Platycladi,3 peaks were from Herba Lycopi,and 2 peaks were from Herba Menthae,there was one new characteristic peak.CONCLUSION:Ten peaks gathered from Shanghai Powder consist of rhein,emodin,chrysophanol,aloe-emodin,physcion,gallic acid,berberine hydrochloride,palmatine;quercitrosid,and linarin.