The effects of physiological ischemia training on endothelial progenitor cells / 中华物理医学与康复杂志

ABSTRACT

Objective To explore the effects of physiological ischemia training (PIT) on the function of endothelial progenitor cells.Methods Eighteen male New Zealand rabbits (2.5 ± 0.5) kg were divided into 3 groups:a sham operation (SO) group,a myocardial ischemia (MI) group and a MI ± physiological ischemia training (MI ± PIT) group.The PIT was modeled by ischemic isometric contraction of gastrocnemius muscle induced by electric stimulation (40 Hz,1 ms,40％ maximum current strength),and the MI was modeled by implantation of a water balloon to occlude the left ventrical branch of the heart.The experiment was conducted for 4 weeks.Before and after the experiments,the ability of mobilization and adhesion of endothelial progenitor cells were detected,the number and the capillary density were counted.Results Post-experiment,compared with groups SO and MI,PIT group significantly improved the ability of mobilization (151 ± 16 cells/HPF,P ＜ 0.01) and adhesion (17.8 ± 2.8 cells/ HPF,P ＜ 0.05),increased the circulating amount (P ＜ 0.05) and capillary density (824.0 ± 106.6 /mm2,P ＜ 0.05).Pearson analyze showed that there was positive correlation between circulating EPCs and capillary density.Conclusion Physiological ischemia training could improve EPCs mobilization and function,resulted to neovascularization in the ischemic heart tissues.