Evaluation of a New Immunochromatographic Assay Kit for the Rapid Detection of Norovirus in Fecal Specimens
Annals of Laboratory Medicine
; : 79-81, 2012.
Article
in En
| WPRIM
| ID: wpr-43982
Responsible library:
WPRO
ABSTRACT
Rapid and accurate detection of norovirus is essential for the prevention and control of norovirus outbreaks. This study compared the effectiveness of a new immunochromatographic assay kit (SD BIOLINE Norovirus; Standard Diagnostics, Korea) and real-time reverse transcription-PCR (RT-PCR) for detecting norovirus in fecal specimens. Compared with real-time RT-PCR, the new assay had sensitivity, specificity, positive predictive value, and negative predictive value of 76.5% (52/68), 99.7% (342/343), 98.1% (52/53), and 95.5% (342/358), respectively. The sensitivity of the assay was 81.8% (18/22) for GII.3 and 75.7% (28/37) for GII.4. None of the 38 enteric virus-positive specimens (3 for astrovirus, 5 for enteric adenovirus, and 30 for rotavirus) tested positive in the cross-reactivity test performed by using this assay. The new immunochromatographic assay may be a useful screening tool for the rapid detection of norovirus in sporadic and outbreak cases; however, negative results may require confirmatory assays of greater sensitivity.
Key words
Full text:
1
Database:
WPRIM
Main subject:
Reagent Kits, Diagnostic
/
RNA, Viral
/
Immunoassay
/
Acute Disease
/
Sensitivity and Specificity
/
Caliciviridae Infections
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Reverse Transcriptase Polymerase Chain Reaction
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Norovirus
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Feces
/
Gastroenteritis
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Adolescent
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Adult
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Aged
/
Aged80
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Child
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Child, preschool
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Humans
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Infant
Language:
En
Journal:
Annals of Laboratory Medicine
Year:
2012
Document type:
Article