Biocompatibility of hydroxyapatite/TiO2 nanotube composites / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
; (53): 335-340, 2014.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-443695
Responsible library:
WPRO
ABSTRACT
BACKGROUND:
Hydroxyapatite has excellent biocompatibility, but biocompatibility of nano-hydroxyapatite/TiO2 nanotube composites is rarely reported.OBJECTIVE:
To evaluate the biocompatibility of nano-hydroxyapatite/TiO2 nanotube composites.METHODS:
First, the TiO2 nanotubes were fabricated on the surface of the titanium by anodic oxidation technique. Second, the nano-hydroxyapatite/TiO2 nanotube composites were fabricated by electrodeposition technique. The surface morphology of the composites was observed by scanning electron microscopy. Mouse osteoblasts MC-3T3-E1 were co-cultured with the nano-hydroxyapatite/TiO2 nanotube composites, TiO2 nanotubes and titanium, and commercial y pure titanium to observe the celladhesion, proliferation and necrosis on scaffolds. RESULTS ANDCONCLUSION:
The morphology of the TiO2 nanotubes and nano-hydroxyapatite/TiO2 nanotube composites could be control ed by altering the conditions of the anodic oxidation and electrodeposition. Under the inverted microscope, after 3 days of co-culture with TiO2 nanotubes and nano-hydroxyapatite/TiO2 nanotube composites, MC-3T3-E1 cells proliferated wel with regular shape and arrangement that were superior to those on commercial y pure titanium. Under scanning electron microscope, the cellwere adhered and proliferated wel on the surface of the TiO2 nanotubes and nano-hydroxyapatite/TiO2 nanotube composites after 3 days. Apoptosis rate of the cells was significantly reduced on the surface of nano-hydroxyapatite/TiO2 nanotube composites (7.8%) compared with TiO2 nanotubes (9.4%) and commercial y pure titanium (13.5%), indicating nano-hydroxyapatite/TiO2 nanotube composites have good biocompatibility.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Tissue Engineering Research
Year:
2014
Document type:
Article