Identification of Tumor Suppressor Genes on Chromosome 21 / 대한흉부외과학회지
The Korean Journal of Thoracic and Cardiovascular Surgery
; : 141-147, 2009.
Article
in Ko
| WPRIM
| ID: wpr-44393
Responsible library:
WPRO
ABSTRACT
BACKGROUND: We performed this study to identify the tumor suppressor genes located in the long arm of chromosome 21 in non-small cell lung cancer. MATERIAL AND METHOD: The genes of USP25 in 21q11.2, NCAM2, ADAMTS1 in 21q21.2, and Claudin-8 (CLDN8), Claudin-17 (CLDN17) and TIAM1 in 21q22.1 were investigated for their gene expressions, genetic alterations and promoter methylation. RESULT: The expressions of CLDN8 and CLDN17 were significantly decreased in 7 (L132, H157, H358, H522, H1299, H1703 and HCC2108) of 13 cell lines, and the expression of ADAMTS1 was also significantly reduced in 6 cell lines (A549, SW900, H1299, H1373, H1703 and H1793). There were no genetic alterations by PCR-SSCP and cDNA cloning in the cell lines with a decreased gene. In the cell lines with a decreased gene expression, the mRNA expression was increased significantly with treatment of 5-Aza-CdR. CONCLUSION: These results suggest that the ADMTS1, CLDN8 and CLDN17 may act as tumor suppressor genes.
Key words
Full text:
1
Database:
WPRIM
Main subject:
Arm
/
Chromosomes, Human, Pair 21
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RNA, Messenger
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Gene Expression
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Cell Line
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Genes, Tumor Suppressor
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Clone Cells
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DNA, Complementary
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Carcinoma, Non-Small-Cell Lung
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Cloning, Organism
Type of study:
Diagnostic_studies
Language:
Ko
Journal:
The Korean Journal of Thoracic and Cardiovascular Surgery
Year:
2009
Document type:
Article