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Determination of Berberine Hydrochloride and Phellodendrine in Lishukang Capsules by HPLC / 中国中医药信息杂志
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-443954
Responsible library: WPRO
ABSTRACT
Objective To establish a method for the determination of berberine hydrochloride and phellodendrine in Lishukang Capsules by HPLC. Methods Waters Symmetry C18 column (4.6 mm× 250 mm, 5μm) was used, the mobile phase was acetonitrile-0.1%phosphoric acid (0.1 g sodidum dodecyl sulfonate in 100 mL water), with the flow rate of 1 mL/min by gradient elution. The detection wavelength was set at 284 nm. Results Berberine hydrochloride showed good linear relationship (r 2=0.999 1) in the range of 403.4-10 084.7 μg, the average recovery rate was 99.8%(RSD=1.7%). Phellodendrine showed good linear relationship (r 2=0.999 2) in the range of 169.137 6-4228.44 μg, the average recovery rate was 95.5%(RSD=3.2%). Conclusion The method is specific, accurate and convenient to be used for the quality control of Lishukang Capsules.

Full text: Available Database: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Information on Traditional Chinese Medicine Year: 2014 Document type: Article
Full text: Available Database: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Information on Traditional Chinese Medicine Year: 2014 Document type: Article
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