The Identification of Uygur Medicine Juniperus sabina by DNA Barcode / 世界科学技术-中医药现代化

ABSTRACT

To establish the DNA barcode identification method of Uygur Medicine Juniperus sabina. Leaves and fruits were selected randomly from different areas to contrast morphology; Ten samples were amplified by ITS2 se-quence, combining with four ITS sequences of Platycladus orientalis obtained from GenBank, intraspecific and inter-specific variation were compared, K2P and NJ methods were utilized for analysising the relationship. The results show J. sabina cannot be distinguished with its adulterants by morphological characteristics of leaves and fruits; 14 Samples of ITS2 sequence length are 219 bp, the maximum K2P distance are 0.089. J. sabina and its 3 adulterants plant can be distinguished by the DNA barcode ITS2 sequence and NJ tree successfully. Therefore, ITS2 barcode sequence was able to identify J. sabina and its adulterants species, which provides an effective way for the molecular identification of Uygur medicine J. sabina.