Molecular Identification of Serpentis Periostracum and Its Adulterants Based on COI Sequence / 世界科学技术-中医药现代化
World Science and Technology-Modernization of Traditional Chinese Medicine
; (12): 284-287, 2014.
Article
in Zh
| WPRIM
| ID: wpr-446430
Responsible library:
WPRO
ABSTRACT
Objective: This study aimed to distinguish Serpentis Periostracum from its adulterants, which will provide the basis for its safe application. Methods: Here, COI sequences of 68 samples from 13 species were PCR amplified and sequenced. Furthermore, the DNA Barcoding Gap and phylogenetic cluster analysis were carried out. Results:The results exhibited that the COI sequences of all the three origin animals of Serpentis Periostracum have DNA Barcoding Gap. For phylogenetic cluster analysis, all the three origin animals showed monophyletic and every species can be discriminated clearly. Conclusion: COI is an effective DNA barcode for the identification of Serpen-tis Periostracum.
Full text:
1
Database:
WPRIM
Type of study:
Diagnostic_studies
Language:
Zh
Journal:
World Science and Technology-Modernization of Traditional Chinese Medicine
Year:
2014
Document type:
Article