The application value of HPV E6/E7 mRNA detection in paraffin-embedded samples for cervical cancer screening / 肿瘤研究与临床
Cancer Research and Clinic
; (6): 243-245, 2014.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-447305
Responsible library:
WPRO
ABSTRACT
Objective To explore the application value of detecting HPV E6/E7 mRNA in cervical cancer screening and prognosis.Methods bDNA technology was used to detect the HPV E6/E7 mRNA levels in 301 cases of paraffin-embedded cervical samples that were grouped based on pathological diagnosis.Statistic differece between multiple groups were assessed by chi-square segmentation method.Results The positive detection rate of HPV E6/E7 mRNA is higher in cervical cancer group than that in CIN1-3 groups and cervicitis group,which were 94.29 % (66/70),30.30 % (20/66),50.00 % (19/38),59.46 % (44/74),5.66 % (3/50),respectively (all P < 0.004 5).The HPV E6/E7-positive rates in all three CIN groups were higher than that in cervicitis group (P < 0.004 5).The positive rates in CIN2 and CIN3 group were higher than that in CIN1 group (P < 0.004 5).HPV E6/E7 mRNA levels in cervical cancer group were higher than those in CIN groups and cervicitis group (16 508.730±8 741.402,929337±460.880,3 239.681±1 447.399,5 286.224±2 933.445,4.941±2.263,respectively,all P < 0.05).CIN groups had higher copy numbers of HPV E6/E7 mRNA than cervicitis group (P < 0.05).More copies of HPV E6/E7 mRNA were detected in CIN3 group than those in CIN1 group (P < 0.05).Conclusions The positive rate and expression levels of HPV E6/ E7 mRNA was related to the severity of cervical diseases.HPV E6/E7 mRNA detection in paraffin-embedded samples have a high clinical application value in predicting the risk of cervical cancer.It' s an effective measure for cervical cancer screening and follow-up.
Full text:
Available
Database:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
/
Prognostic study
/
Screening study
Language:
Chinese
Journal:
Cancer Research and Clinic
Year:
2014
Document type:
Article