Plasma G-CSF and GM-CSF Concentrations and Expression of their Receptors on the Granulocyte in Children with Leukocytosis
Journal of the Korean Pediatric Society
; : 271-276, 2003.
Article
in Korean
| WPRIM (Western Pacific)
| ID: wpr-44750
Responsible library:
WPRO
ABSTRACT
PURPOSE:
Granulocyte-colony stimulating factor(G-CSF) and granulocyte macrophage-colony stimulating factor(GM-CSF) are principal cytokines in granulopoiesis and their physiologic effects are mediated through binding to specific cell surface receptors. Although it is known that the level of serum G-CSF and GM-CSF, and presentation of the receptors are increased in infectious diseases, there have been no studies to find the correlation between the granulopoiesis and leukocytosis. This study was designed to measure G-CSF and GM-CSF in leukocytosis and in control and to demonstrate the possible pathogenesis of granulopoiesis in leukocytosis using quantitative analysis of G- CSF, GM-CSF and their CSFr.METHODS:
The plasma levels of G-CSF, GM-CSF of 13 children without leukocytosis and 14 children with leukocytosis were measured. Counts of cell surface G-CSFr and GM-CSFr were measured by combining anti G-CSFr and anti GM-CSFr monoclonal antibodies to their respective receptors by using quantitative flow cytometric assay.RESULTS:
There was no significant difference betweeen the plasma concentration of G-CSF and GM-CSF in acute leukocytosis and in the control group. However, levels of G-CSFr in acute leukocytosis decreased significantly compared to the control(P=0.012) and the levels of GM-CSFr in both groups revealed no significant difference.CONCLUSION:
Increase in the number of leukocyte in leukocytosis was mediated by increasing the number of neutrophil, and increased plasma concentration of G-CSF may be the cause of neutrophilia. But GM-CSF did not have any influence on leukocytosis.
Full text:
Available
Health context:
SDG3 - Health and Well-Being
Health problem:
Target 3.3: End transmission of communicable diseases
Database:
WPRIM (Western Pacific)
Main subject:
Plasma
/
Communicable Diseases
/
Cytokines
/
Granulocyte Colony-Stimulating Factor
/
Granulocyte-Macrophage Colony-Stimulating Factor
/
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor
/
Receptors, Granulocyte Colony-Stimulating Factor
/
Receptors, Cell Surface
/
Granulocytes
/
Leukocytes
Limits:
Child
/
Humans
Language:
Korean
Journal:
Journal of the Korean Pediatric Society
Year:
2003
Document type:
Article