Characterization and comparison of interferon reference standards using UPLC-MS / 药学学报
Acta Pharmaceutica Sinica
; (12): 75-80, 2015.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-457215
Responsible library:
WPRO
ABSTRACT
The study aims to characterize and compare interferon reference standards from 5 manufacturers. By testing molecular mass and trypsin-digested peptide mass mapping, the amino acid sequence was verified and post-translational modifications such as disulfide bond were identified. Results show that the molecular mass and amino acid sequence were consistent with theory; the disulfide bonds of 4 lots of interferon were Cys1-Cys98/Cys29-Cys138, 1 lot was Cys29-Cys139/Cys86-Cys99; N-terminal "+Met", acetyl N-terminal and Met oxidation were identified in part of the sample. UPLC-MS can be used to characterize and compare interferon reference standards from different manufacturers.
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Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2015
Document type:
Article