Benzoxazole Derivative B-98 Ameliorates Dextran Sulfate Sodium-induced Acute Murine Colitis and the Change of T Cell Profiles in Acute Murine Colitis Model / 대한소화기학회지
The Korean Journal of Gastroenterology
; : 33-41, 2013.
Article
in Korean
| WPRIM (Western Pacific)
| ID: wpr-46505
Responsible library:
WPRO
ABSTRACT
BACKGROUND/AIMS:
The unique role of enzyme 5-lipoxygenase (5-LO) in the production of leukotrienes makes it a therapeutic target for inflammatory bowel disease (IBD). The aim of this study was to evaluate the effects of B-98, a newly synthesized benzoxazole derivatives and a novel 5-LO inhibitor, in a mouse model of IBD induced by dextran sulfate sodium (DSS).METHODS:
C57BL/6 mice were randomly assigned to four groups normal control, DSS colitis (DSS+saline), low dose B-98 (DSS+B-98 20 mg/kg) and high dose B-98 (DSS+B-98 100 mg/kg). B-98 was administered with 3% DSS intraperitoneally. The severity of the colitis was assessed via the disease activity index (DAI), colon length, and histopathologic grading. The production of inflammatory cytokines interleukin (IL)-6 was determined by RT-PCR. Th cells were examined for the proportion of Th1 cell, Th2 cell, Th9 cell, Th17 cell and Treg cell using intracellular cytometry.RESULTS:
The B-98 group showed lower DAI, less shortening of the colon length and lower histopathologic grading compared with the DSS colitis group (p<0.01). The expression of IL-6 in colonic tissue was significantly lower in the B-98 groups than the DSS colitis group (p<0.05). The cellular profiles revealed that the Th1, Th9 and Th17 cells were increased in the DSS colitis group compared to the B-98 group (p<0.05).CONCLUSIONS:
Our results suggest that acute intestinal inflammation is reduced in the group treated with B-98 by Th1, Th9 and Th17 involved cellular immunity.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Benzoxazoles
/
Severity of Illness Index
/
Arachidonate 5-Lipoxygenase
/
T-Lymphocytes
/
Acute Disease
/
Dextran Sulfate
/
Interleukin-6
/
Lipoxygenase Inhibitors
/
Colitis
/
Colon
Type of study:
Prognostic study
Limits:
Animals
Language:
Korean
Journal:
The Korean Journal of Gastroenterology
Year:
2013
Document type:
Article