Preparation of a murine model of systemic Candida albicans infection / 中国实验动物学报
Acta Laboratorium Animalis Scientia Sinica
; (6): 301-305, 2015.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-467395
Responsible library:
WPRO
ABSTRACT
To establish a stable mouse model of systemic Candida infection and to set up related standard operation procedure .Methods ICR mice were infected with C.albicans or C.parapsilosis by tail vein injection after immunosuppres-sion by cyclophosphamide .The quality control key points included immunosuppression , strain preparation, inoculation doses and the route of inoculation .Survival analysis , bacterial loads and pathological examination were performed to evaluate the prepared model .Results The developed model showed fugal-specific lesions in multiple organs , especially in the kidneys revealed by histopathological examination .Conclusions A stable mouse model of systemic Candida albicans infection can be successfully established by following standardized operation procedure .This mouse model may provide a useful tool for studies on pathogenesis and immune defense of fungal infection and new anti-fungal drug development and so on .
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Acta Laboratorium Animalis Scientia Sinica
Year:
2015
Document type:
Article