Effect of propofol post-conditioning on ADAR2-AMPA receptor GluR2 pathway in hippocampal neurons of fetal rats subjected to oxygen-glucose deprivation / 中华麻醉学杂志

ABSTRACT

Objective To investigate the effect of propofol post-conditioning on RNA2 (ADAR2)-α-amino-3-hydroxy-5-methyliso xazole-4-propionic acid (AMPA) receptor subunit glutamate 2 (GluR2) pathway in hippocampal neurons of fetal rats subjected to oxygen-glucose deprivation (OGD).Methods The hippocampal neurons were isolated from the fetal rats obtained from Wistar rats at 16-18 days of gestation and primarily cultured for 7 days.The primarily cultured neurons were randomly divided into 3 groups (n =6 each)control group (group C) ; OGD group (group O) ; propofol post-conditioning group (group P).The cells were subjected to OGD for 1 h followed by restoration of O2-glucose supply in group O.In group P,the cells were subjected to OGD for 1 h followed by restoration of O2-glucose supply and then 1.2 μg/ml propofol was added and the cells were cultured for 2 h and then the culture medium was replaced with plain culture medium.At 24 h of incubation,the cells were collected for assessement of the survival rates of the hippocampal neurons and for determination of the expression of ADAR2 mRNA (by RT-PCR),the total ADAR2 protein (tADAR2) and ADAR2 protein in the nucleus of cells (nADAR2) (by Western bolt).The editing percentage of GluR mRNA at the Q/R site was analyzed by nest RT-PCR and BbV1.Results There was no significant difference in the expression of ADAR2 mRNA and tADAR2 among the three groups.Compared with group C,the survival rates of the hippocampal neurons were significantly decreased,the expression of nADAR2 was down-regulated,the ratio of nADAR2/tADAR2 was decreased,and the editing percentage of GluR mRNA at the Q/R site was decreased in group O.Compared with group O,the survival rates of the hippocampal neurons were significantly increased,the expression of nADAR2 was up-regulated,the ratio of nADAR2/tADAR2 was increased,and the editing percentage of GluR mRNA at the Q/R site was increased in group P.Conclusion Propofol post-conditioning reduces OGD-induced damage to hippocampal neurons of fetal rats through activating ADAR2-AMPA receptor GluR2 pathway.