Role of Inhibitory Transforming Growth Factor-β Signal Smad7 in Helicobacter pylori-associated Gastric Damage / 대한소화기학회지
The Korean Journal of Gastroenterology
; : 186-194, 2016.
Article
in Ko
| WPRIM
| ID: wpr-47258
Responsible library:
WPRO
ABSTRACT
BACKGROUND/AIMS: Transforming growth factor-beta (TGF-β) is a cytokine implicated in the susceptibility, development, and progression of gastrointestinal cancer and certain other neoplasms. In the later stages of cancer, TGF-β not only acts as a bystander of host-immune response, but also contributes to cell growth, invasion, and metastasis. In the current study, we generated gastric mucosal cells that stably express Smad7, and explored the Helicobacter pylori-associated biological changes between mock-transfected and Smad7-transfected RGM1 cells. METHODS: RGM1 cells stably transfected with Smad7 were infected with H. pylori, and molecular changes in apoptotic markers and inflammatory mediators were examined. Several candidate genes were explored in Smad7-overexpressing cells after H. pylori infection. RESULTS: Overexpression of Smad7 in RGM1 cells significantly increased the H. pylori-induced cytotoxicity compared to mock-transfected cells. Exaggerated increases in inflammatory mediators, cyclooxygenase 2, inducible NO synthase, and augmented apoptosis were noted in Smad7-overexpressing cells, whereas mitigated heme oxygenase 1 was noted in Smad7- overexpressing cells. These phenomena were reversed in cells transfected with Smad7 siRNA. CONCLUSIONS: These data suggest that inhibition of Smad7 is a possible target for mitigating H. pylori-associated inflammation.
Key words
Full text:
1
Database:
WPRIM
Main subject:
Transforming Growth Factor beta
/
Helicobacter pylori
/
Helicobacter
/
Apoptosis
/
Nitric Oxide Synthase
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RNA, Small Interfering
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Cyclooxygenase 2
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Heme Oxygenase-1
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Gastritis
/
Gastrointestinal Neoplasms
Language:
Ko
Journal:
The Korean Journal of Gastroenterology
Year:
2016
Document type:
Article