Metformin improving anxiety-like behaviors through regulation of autophagy following focal cerebral ischemia in rats / 中国生化药物杂志

ABSTRACT

Objective To investigate probable effect of metformin on anxiety and autophagy pathway in rats with focal cerebral ischemia . Methods The rats were divided into seven groupsshame group, ischemia group, metformin group, compound C group, compound C +ischemia group, metformin+ischemia group, and metformin+compound C+ischemia group.The rats were administered with metformin 14 days and compound C 30 min pretreatment.The blood glucose level, body mass, neurological score and indicators of elevate plus maze test were recorded of two groups after 72h ischemia-perfusion.The autophagy-related protein of Atg-7, Atg-5, beclin-1 were analysed by Western blot.ResuIts Metformin had no significant effect of body mass and blood glucose level.The prior administration of metformin significantly elevated neurological score in ischemia group (P<0.01).The elevate plus maze test results showed that prior administration of metformin significantly elevated percentage of open-arms detention time in ischemia group (P<0.01), but there was no significant difference in open-arms times.Western blot result showed that prior administration of metformin significantly elevated Atg-7, Atg-5, beclin-1 protein expression in ischemia group (P<0.001).Compound C resist the above effects of metformin (P<0.01).ConcIusion The metformin improves psychological disorders and movement disability through regulation of autophagy following focal cerebral ischemia in rats.