Impact of different mouse blastocyst donors on the efficiency of germline chimera production from C57BL/6 embryonic stem cells / 中国实验动物学报
Acta Laboratorium Animalis Scientia Sinica
; (6): 353-358, 2015.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-477294
Responsible library:
WPRO
ABSTRACT
Objective To improve the gene targeting efficiency with C57BL/6 embryonic stem ( ES) cells.Meth-ods Three different genetically modified C57BL/6 ES cell lines, named TLX3, Ai3K and SL, were microinjected into ICR, B6( Cg)-Tyrc-2J and BALB/c mouse blastocysts, respectively.The efficiency was statistically evaluated according to three aspectsblastocyst collection, chimera production and germline transmission.Results None of the three ES cell lines was germline transmitted with B6(Cg)-Tyrc-2J mice as blastocyst donors, while it was achieved with both BALB/c and ICR mouse blastocysts.Compared in the aspect of blastocysts collection, ICR mouse was much better than BALB/c mouse (P<0.05), and the chimera production efficiency of ICR mouse was comparable to that of BALB/c mouse (P =0.115). As to the germline transmission efficiency, that of BALB/c mice is significantly higher than that of the ICR mice ( P<0.01).Conclusions The germline transmission efficiency of BALB/c mouse is highest among these three mouse strains. However, it has the disadvantages of blastocyst collection, developmental delay and zona pellucida fragility, compared with ICR mouse.Therefore, ICR mouse is also a good candidate as blastocyst donor for embryonic stem cell microinjection.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Acta Laboratorium Animalis Scientia Sinica
Year:
2015
Document type:
Article