Mechanism by which interferon reduces the resistance of MGMT positive glioma stem cells to temozolomide / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
; (53): 5800-5805, 2015.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-477481
Responsible library:
WPRO
ABSTRACT
OBJECTIVE:
To investigate the role of interferon to increase the sensibilization of MGMT positive glioma stem cel s to temozolomide in vitro.METHODS:
Glioma cel lines, U251 and SKMG-4, were induced by suspended cloning bal formation method to harvest MGMT positive glioma stem cel s, U251G and SKMG-4G. Cel counting kit-8 assay was used to detect the kil ing effect of interferonα/βcombined with temozolomide on MGMT positive glioma stem cel s. RT-PCR and western blot assay were employed to determine the expression of MGMT and nuclear factorκB in MGMT positive glioma stem cel s. RESULTS ANDCONCLUSION:
Western blot results showed positive expression of MGMT in U251G and SKMG-4G cel s at protein levels. After intervention with interferonα/β, the mRNA expression of MGMT and nuclear factorκB in SKMG-4G and U251G cel s was reduced significantly, and then further decreased after temozolomide treatment. These findings indicate that interferonα/βcan remarkably strengthen the kil ing effect of temozolomide on MGMT positive glioma stem cel s.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Tissue Engineering Research
Year:
2015
Document type:
Article