Clinical significance of enzyme linked immunosorbent assay and indirect immunofluorescence for detection of antinuclear antibody / 国际检验医学杂志

ABSTRACT

Objective To compare the value of ELISA and indirect immunofluorescence (IIF)method in diagnosis for autoim-mune diseases.Methods A total of 33 patients in systemic lupus erythematosus(SLE)group,59 patients in other autoimmune dis-eases group,43 patients in non-autoimmune disease group,20 people accepted physical examination in control group.The antinuclear antibody (ANA)in each group were detected by two methods and analyzed.Results The high titer ANA detected by ELISA and IIF in SLE group and other autoimmune diseases group were (2.621±1.700),(2.248±1.781);(2.71 5±0.730),(2.544±0.59). The titer ANA detected by ELISA in non-autoimmune disease group was (1.034±1.050),which was lower than(2.253 ±0.691) detected by IIF.Conclusion ELSA might improve the detection effects of ANA antibody.