Effects of White Mange Mixture on VEGF Content and Genetic and Protein Expressions of VEGFR-2 in Cultured HaCaT Cells / 中国中医药信息杂志

ABSTRACT

Objective To observe the effects of White Mange Mixture on the content of VEGF and the genetic and protein expressions of VEGFR-2 of HaCaT cells;To explore its mechanism. Methods HaCaT cells were cultured in vitro to establish psoriasis experimental model. SD rats were randomly divided into the blank serum group, white mange mixture of low-, medium- and high- dose groups and acitretin group, meanwhile to set up blank control group. Cultured HaCaT cells model were intervened with different medicine serum. ELISA was applied to detect the content of VEGF. RT-PCR and Western blot were used to detect the genetic and protein expressions of VEGFR-2.Results Compared with the blank control group, White Mange Mixture of low-, medium- and high-dose groups interventing HaCaT cells could obviously inhibit the secretion of VEGF and decrease the genetic and protein expression of VEGFR-2 in a dose-dependent manner. Conclusion White Mange Mixture plays a role in the treatment of psoriasis possibly through inhibiting the content of VEGF and decreasing the genetic and protein expressions of VEGFR-2.