Uptake Property Study on Punicalagin in Caco-2 Cells / 中国中医药信息杂志

ABSTRACT

Objective To study the uptake property of punicalagin in Caco-2 cells.Methods Caco-2 cell monolayer model was used to research the effects of punicalagin on cell uptake in different times, concentrations, pH values, temperatures and P-gp inhibitors. HPLC was used to determine the content of punicalagin in cells.Results The linearity of punicalagin concentration curve was found in the range of 0.024 8-0.992μg (r=0.997 9). Uptake determination of punicalagin in Caco-2 cells absorbed a certain amount of time, concentration and pH dependence and P-gp inhibitors could significantly increase the punicalagin uptake.Conclusion This method is simple and sensitive to determine the punicalagin concentration in Caco-2 cells, which is mainly passively transported. P-gp is involved in punicalagin transport process.