Microglial P2X7 receptor expression is accompanied by neuronal damage in the cerebral cortex of the APPswe/PS1dE9 mouse model of Alzheimer's disease
Experimental & Molecular Medicine
; : 7-14, 2011.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-48419
Responsible library:
WPRO
ABSTRACT
The possibility that P2X7 receptor (P2X7R) expression in microglia would mediate neuronal damage via reactive oxygen species (ROS) production was examined in the APPswe/PS1dE9 mouse model of Alzheimer's disease (AD). P2X7R was predominantly expressed in CD11b-immunopositive microglia from 3 months of age before Abeta plaque formation. In addition, gp91phox, a catalytic subunit of NADPH oxidase, and ethidium fluorescence were detected in P2X7R-positive microglial cells of animals at 6 months of age, indicating that P2X7R-positive microglia could produce ROS. Postsynaptic density 95-positive dendrites showed significant damage in regions positive for P2X7R in the cerebral cortex of 6 month-old mice. Taken together, up-regulation of P2X7R activation and ROS production in microglia are parallel with Abeta increase and correlate with synaptotoxicity in AD.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Aging
/
Mice, Transgenic
/
Receptors, Immunologic
/
Gene Expression
/
Cerebral Cortex
/
Blotting, Western
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Amyloid beta-Peptides
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Reactive Oxygen Species
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Microglia
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Plaque, Amyloid
Limits:
Animals
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2011
Document type:
Article