Clarification Process of Mulberry Leaf Extracts with ZTC1+1Ⅱ Natural Clarifying Agent / 医药导报

ABSTRACT

Objective To determine the optimum process of ZTC1+1Ⅱnatural clarifying agents edulcorated mulberry leaf extracts. Methods The remaining rate of mulberry leaf alkaloid,flavone,polysaccharide and the solids removal rate were used as indexes,optimized by single factor experiment to study the effect of some factors,such as the order and dosage of clarifying agents,the concentration and the temperature of the extracting solution,whisking time,whisking speed and holding time,response surface methodology was selected to decide the best clarfication process. Results The optimal processthe order of the ZTC1+1Ⅱ natural clarifying agents was part B after part A,the dosage of clarifiers B and A were 10% and 5%volume of the extract,the extract solution was 0.17 g·mL-1 ,the temperature was 76 ℃,the whisking speed was 120 r·min-1 , the whisking time was 10 min,80 ℃ holding 30 min,and standing time was 12 h,the remaining rate of mulberry leaf alkaloid, flavone and polysaccharide were 92.5%,90.2% and 91.1%,the solids removal rate was 26.5%. Conclusion ZTC1+1Ⅱnatural clarifying agents could effectively purify the extracts of mulbery leaf,optimized by the response surface method,the method is simple and feasible,and clarity is good.