Content Determination of Glucosamine Hydrochloride and its Preparations by HPLC / 中国药师
China Pharmacist
; (12): 387-389, 2016.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-487033
Responsible library:
WPRO
ABSTRACT
Objective:
To establish an HPLC method for determining the content of glucosamine hydrochloride and its prepara-tions.Methods:
A Phenomenex Luna NH2 column was used, and the mobile phase was ammonium phosphate buffer (dissolving 3. 5 g dipotassium phosphate and 0. 25 ml ammonium hydroxide into 1 000 ml water, adjusting pH to 7. 5 with phosphoric acid) – acetoni-trile (25 ∶75). The flow rate was 1. 5 ml·min-1 and detection wavelength was 195 nm. The column temperature was 35℃ and the injection volume was 20 μl.Results:
The linear range of glucosamine hydrochloride was 1. 6-16. 0 mg·ml-1(r=0. 999 9). The av-erage recovery of glucosamine hydrochloride tablets and capsules was 99. 3% and 99. 5%, respectively, and RSDs were 0. 3% ( n=9).Conclusion:
The method is simple, accurate and reliable, and suitable for the quality control of glucosamine hydrochloride and its preparations.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2016
Document type:
Article