The studying of fosinopril combined with fenofibrate on the preventing of diabetic retinopathy in diabetic mice / 天津医药

ABSTRACT

Objective To study the effect and mechanism of the Fosinopril combined with Fenofibrate on the prevent?ing of diabetic retinopathy. Methods A total of 150 viripotent ICR mice(100 male mice, 50 female mice) were randomly di?vided into five groups(n=30), including A group (Sham group), B group (Model group), C group [Fosinopril prevented group, 20 mg/(kg·d)], D group [Fenofibrate prevented group, 400 mg/(kg·d)] and E group (Fosinopril combined with Fenofibrate pre?vented group). The expression levels of Bax and Bcl-2 gene mRNA were determined by RT-PCR method. TUNEL staining method was used to detect the apoptosisi of retinal cells. Results The Bcl-2 mRNA of A group, Bax mRNA of B group were higher than those of other four groups. Bcl-2 mRNA of E group was higher than that of C group and D group, while the Bax mRNA was lower than those of two groups(all P<0.05). The TUNEL index of B group was the highest than other groups, which of E groupsimilar. The activi?ties of GSH-PX and SOD of B group were lower than those of other four groups, while the levels of ROS, MDA, VEGF were higher than those of them, the activity of GSH-PX and SOD of E group were higher than those of C and D groups, while the levels of ROS, MDA, VEGF were lower than them(all P<0.05). Conclusion Fosinopril and Fenofibrate can improve dia?betic retinopathy through inhibiting the apoptosis and antioxidation. The combination of them can get even better effect.