Simultaneous Determination of Hydroxysafflor Yellow A and Aalvianolic Acid B in Compound Danshen Oral Solution by HPLC / 中国药房
China Pharmacy
; (12): 4726-4728, 2015.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-500883
Responsible library:
WPRO
ABSTRACT
OBJECTIVE:
To establish a method for simultaneous determination the contents determination of hydroxysafflor yel-low A and salvianolic acid B in Compound danshen oral solution.METHODS:
HPLC was performed on the column of SHIMADZU ODS-C18 with mobile phase of methanol-0.5% phosphoric acid (3565,V/V) at flow rate of 1.0 ml/min,dection wavelength was 403 nm for hydroxysafflor yellow A and 286 nm for salvianolic acid B and column temperature was 35 ℃, the volume injection was 20μl.RESULTS:
The linear range was 2.01-20.10μg/ml for hydroxysafflor yellow A(r=0.999 9)and 39.80-398.00μg/ml(r=0.999 9) for salvianolic acid B. RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 98.26%-101.25%(RSD=0.94%,n=9)and 98.70%-101.35%(RSD=0.71%,n=9),respectively.CONCLUSIONS:
The method is feasible and reproducible,and can be used for the contents determination of hydroxysafflor yellow A and salvianolic acid B in Com-pound danshen oral solution.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2015
Document type:
Article