Effect of gypenoside on lipopolysaccharide-mediated microglial inflammatory response / 国际脑血管病杂志
International Journal of Cerebrovascular Diseases
; (12): 730-733, 2016.
Article
in Zh
| WPRIM
| ID: wpr-501677
Responsible library:
WPRO
ABSTRACT
Objective To investigate the effect of gypenoside on lipopolysaccharide (LPS)-mediated inflammatory response. Methods The BV2 microglia cell line was cultured in vitro. The BV2 microglia cells were divided into four groups: normal control, LPS (10 ng/ml), GP + LPS (GP 20 μg/ml, LPS 10 ng/ml), and GP (20 μg/ml). After 24 h cultivation, ELISA was used to detect the levels of tumor necrosis factor α(TNF-α), interleukin (IL)-1β, and IL-6. Immunocytochemistry staining and Western blot were used to detect the expression levels of nuclear factor (NF-κB) and suppressor of cytokine signaling 1 (SOCS-1). Results Compared with the normal control group, the release of TNF-α, IL-1β and IL-6, as well as the expression level of NF-κB in the LPS group were increased significantly (all P 0. 05 ). Conclusions GP can significantly inhibit the LPS-mediated microglial inflammatory response. SOCS-1 protein may be involved in GP inhibiting LPS-mediated microglial inflammatory response.
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
International Journal of Cerebrovascular Diseases
Year:
2016
Document type:
Article