5-Azacytidine induces differentiation of bone marrow mesenchymal stem cells into myocardial cells / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
; (53): 7469-7474, 2016.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-508670
Responsible library:
WPRO
ABSTRACT
BACKGROUND:
Bone marrow mesenchymal stem cel s can be differentiated into myocardial cel s induced by 5-azacytidine in vitro, which provides an opportunity for cel transplantation in the treatment of heart disease.OBJECTIVE:
To study the induction effects of 5-azacytidine at different concentrations on the myocardial differentiation of bone marrow mesenchymal stem cel s in rats.METHODS:
Passage 3 bone marrow mesechymal stem cel s were incubated with DMEM containing 0, 5, 10, 20μmol/L 5-azacytidine for 24 hours, and then the induced medium was replaced by DMEM containing 5%fetal bovine serum for subsequent 28-day culture. Afterwards, expression of cardiac troponin I was detected by immunocytochemical method. RESULTS ANDCONCLUSION:
Cel death occurred at 24 hours after 5-azacytidine induction, which was more obvious in the 20μmol/L group than the 5 and 10μmol/L groups. The positive expression of cardiac troponin I was significantly lower in the 5μmol/L group than the 10 and 20μmol/L groups (P0.05). These experimental findings indicate that 5-azacytidine can induce the differentiation of rat bone marrow mesenchymal stem cel s into myocardial cel s in vitro, and its optimal concentration is 10μmol/L.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Tissue Engineering Research
Year:
2016
Document type:
Article