Effect of Fuzheng Jiedu Tongluofang on MMP-2 and MMP-9 and invasion of human hepatoma HepG2 cells / 中国免疫学杂志
Chinese Journal of Immunology
; (12): 542-544, 2017.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-513742
Responsible library:
WPRO
ABSTRACT
Objective:
To investigate the effect and mechanism of Fuzheng Jiedu Tongluofang on the expression of MMP-2,MMP-9 and invasion of human hepatoma HepG2 cell.Methods:
Hepatioma HepG2 cell was treated with Fuzheng Jiedu Tongluofang.The cell viability was measured by CCK8.The invasion of HepG2 was detected by Transwell assay.The expression of MMP-2 and MMP-9 was analyzed by ELISA.Results:
In the CCK8 assay,Fuzheng Jiedu Tongluofang could inhibit the proliferation of HepG2 cells at a density and time measure.In the Transwell assay,the inhibitory rate of invasion was 52.45% when treated with Fuzheng Jiedu Tongluofang at a concentration of 2.65 mg/ml. The ELISA assay indicated that the expression of MMP-2 and MMP-9 decreased significantly after treated with Fuzheng Jiedu Tongluofang(P<0.05).Conclusion:
Fuzheng Jiedu Tongluofang suppressed the invasion of HepG2 with the possible mechanism of down-regulating the expression of MMP-2 and MMP-9 in HepG2.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2017
Document type:
Article