Effect of interleukin-13 on the growth of human erythroleukemia cell line / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12)1986.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-516574
Responsible library:
WPRO
ABSTRACT
In this study we investigated the effect of recombinant human interleukin-13 (IL-13) on the growth of human erythroleukemia cell line(HEL)-a human megakaryobiastic leukemia cell line. HEL cells were cultured in the presence or absence of IL-13. After incubation for 4 days, the cells were first counted and then stained with a direct immunofiuorescence method for flow cytometric analysis of the expression of glycoprotein(GP) Ⅱ b/Ⅲ a. In order to examine the effect of IL-13 on the proliferation of HEL cells, the immunocyte assay was used for the detection of 5-bromo-2'-deoxyuridine (5-Brdu) incorporated into the DNA of HEL cells after they had been cultured for 3 days. We found that the expression of GP Ⅱ b/Ⅲ a on IL-13 treated HEL cells was increased to a significant degree (P
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
1986
Document type:
Article