Establishment of human lymphocyte cell line secreting monoclonal antibodies against Rhesus(D) antigen and sequence analysis of a human monoclonal anti-D Fab fragment / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12)1986.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-518840
Responsible library:
WPRO
ABSTRACT
AIM:
To establish human lymphocyte cell line secreting monoclonal antibody against Rhesus(D) antigen and analyse the nucleotide and deduced amino acid sequences of a human monoclonal anti-D Fab fragment.METHODS:
By using PCR method, the cDNA of human anti-(Rhesus D) antibody(lgM ?)Fab fragment was amplified from an Epstein- Barr-virus-transformed cell line. Cloning and subsequent sequence analysis of the Fab fragment was performed. The deduced amino acid sequence was compared and analysed with previously published sequences.RESULTS:
A band of approximate 700 and 650 base pairs was amplified using lgM heavy chain primers and ? light chain primers, respectively. Sequence analysis indicated that the deduced amino acid sequences was in agreement with the characterization of the amino acid present in the human lg Fab fragment.CONCLUSION:
The cloning and sequencing of a human anti-Rhesus (D) antibody Fab fragment cDNA will make benefits for production of recombinant anti-Rhesus (D) antibody and prevention of Rh haemolytic disease in the newborn.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
1986
Document type:
Article