Rapid Identification of Rickettsiae using the Real-Time PCR
Journal of Bacteriology and Virology
; : 221-226, 2008.
Article
in Korean
| WPRIM (Western Pacific)
| ID: wpr-52015
Responsible library:
WPRO
ABSTRACT
In this study, new real-time PCR method based on the groEL gene was developed and investigated. Four spotted fever group (SFG) strains, four typhus group (TG) strains, and four scrub typhus group (STG) strains were easily differentiated as a distinct entity. This PCR assay was applied to detect Rickettsia DNA from 100 ticks. Twelve Haemaphysalis longicornis ticks were found positive and identified as spotted fever group Rickettsia. This real-time PCR method could simultaneously perform the rapid identification of rickettsiae and the differential diagnosis of SFG, TG, and STG in a single reaction.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Rickettsia
/
Ticks
/
DNA
/
Scrub Typhus
/
Typhus, Epidemic Louse-Borne
/
Polymerase Chain Reaction
/
Diagnosis, Differential
/
Fever
/
Real-Time Polymerase Chain Reaction
Type of study:
Diagnostic study
/
Prognostic study
Language:
Korean
Journal:
Journal of Bacteriology and Virology
Year:
2008
Document type:
Article