T-CAM, a fastatin-FIII 9-10 fusion protein, potently enhances anti-angiogenic and anti-tumor activity via alphavbeta3 and alpha5beta1 integrins
Experimental & Molecular Medicine
; : 196-207, 2008.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-52235
Responsible library:
WPRO
ABSTRACT
We made fusion protein of fastatin and FIII 9-10, termed tetra-cell adhesion molecule (T-CAM) that can interact simultaneously with alphavbeta3 and alpha5beta1 integrins, both playing important roles in tumor angiogenesis. T-CAM can serve as a cell adhesion substrate mediating adhesion and migration of endothelial cells in alphavbeta3 and alpha5beta1 integrin-dependent manner. T-CAM showed pronounced anti-angiogenic activities such as inhibition of endothelial cell tube formation, endothelial cell proliferation, and induction of endothelial cell apoptosis. T-CAM also inhibited angiogenesis and tumor growth in mouse xenograft model. The anti-angiogenic and anti-tumoral activity of molecule like fastatin could be improved by fusing it with integrin-recognizing cell adhesion domain from other distinct proteins. The strategy of combining two distinct anti-angiogenic molecules or cell adhesion domains could facilitate designing improved anticancer agent of therapeutic value.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Benzocaine
/
Recombinant Fusion Proteins
/
Factor VIII
/
Base Sequence
/
Cell Movement
/
Cells, Cultured
/
Chloramphenicol
/
DNA Primers
/
Angiogenesis Inhibitors
/
Integrin alpha5beta1
Type of study:
Prognostic study
Limits:
Animals
/
Humans
/
Male
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2008
Document type:
Article