Selection of targeted glioblastoma tumor cell-binding and internalizing peptides through phage display vector / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12)1989.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-522621
Responsible library:
WPRO
ABSTRACT
AIM:
To isolate peptides targeted binding and internalizing into glioblastoma cell line SWO-38.METHODS:
Tumor cells were screened five rounds of whole cell screen through the Ph.D.-12 phage display library. The monoclone specific binding efficiency to the tumor cell was analyzed, and the DNA of phages were extracted, sequenced and translated to the sequences of amino acid.RESULTS:
In the phage library after five rounds of screen , 10 of 13 monoclones had highly selective binding to SWO-38 cells. We found two repeated peptide sequences.CONCLUSION:
Whole cell screening against tumor cells through random phage peptide library can obtain phage peptides with highly specific binding and internalizing ability. The peptides could be used as a therapy vector for tumor targeted delivery.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
1989
Document type:
Article