Reconstructed corneal endothelial tissue on allo-stroma in vitro / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12)1989.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-522772
Responsible library:
WPRO
ABSTRACT
AIM:
In order to search carrier material and better tissue culture method, the morphology and structure of the cultured cat corneal endothelium was observed.METHODS:
The cat corneal endothelial cells were seeded on the Descemet's membrane of the dehydrated swine corneal stroma, and then cultured in the medium with epidermal growth factor and laminin for 7 days. The morphology and structure of reconstructed tissue was tested by the inverted microscope and the scanning electron microscope.RESULTS:
As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemet's membrane, which was similar to the nature tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately.CONCLUSION:
The cat corneal endothelial cells could be rebuilt on the carrier of the dehydrated swine corneal stroma successfully, which is similar to the nature tissue.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
1989
Document type:
Article