A study on collagen Ⅳ and MMP-2 expression during the period of venous intima hyperplasia (IH) in rat vein grafts / 中国普通外科杂志
Chinese Journal of General Surgery
; (12)2001.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-522877
Responsible library:
WPRO
ABSTRACT
Objective To investigate collagen Ⅳ and matrix metalloproteinase-2 (MMP-2)expression in intimal hyperplasia(IH) in rat vein grafts. Methods Vein graft model was constructed in rats. At day 3,week 1,2,4,8 and 12 after surgery,vein grafts were obtained and examined by HE and Verhoeff stains. The thickness and area ratio of intima/media were measured. The positive cells of 5′-Brdu were detected to indicate SMC proliferation. The positive ratio of collagen Ⅳ and MMP-2′s protein and mRNA were calculated. Results The neointima was found at 1 week post-operation. The maximal IH occurred at 2 to 4 week. The similar tendency was observed in the positive cells of 5′-Brdu. Collagen Ⅳ was on the decrease at 1 week and negative from 2 to 8 week ( P
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of General Surgery
Year:
2001
Document type:
Article