The Effect of CGRP on Free Calcium Concentration in Brain Cells of Hypoxic-ischemic Neonatal SD Rats / 中国医师杂志

ABSTRACT

Objective To explore the effect of calcium gene-related peptide (CGRP) on free calcium concentration in the brain cells of hypoxic-ischemic neonatal SD rats. Methods Animal model of hypoxic-ischemic cerebral injury was set up using SD rats of 7 days old. Then the rats were randomly divided into treatment group given 3?g/kg/d CGRP intraperitoneally for 3 days immediately after the model was made, and salt solution group given 0.9% NaCl solution intraperitoneally for 3 days. Normal control group received sham operation. All the rats were decapitated after 3 days and the concentration of free calcium in brain cells was measured with calcium fluorescence indicator in Fura-2Am. Results The free calcium concentration in brain cells in salt solution group was significantly higher than that in the other two groups (P