The in vitro immune effects of dendritoma formed by mouse hepatocellular carcinoma cells and lymphotactin gene modified dendritic cells / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12)1986.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-524695
Responsible library:
WPRO
ABSTRACT
AIM:
To investigate the in vitro anti tumo r immune responses of dendritoma formed by mouse hepatocellular carcinoma cells and lymphotactin gene modified dendritic cells (DCs).METHODS:
DCs prepared from mouse bone marrow were genetically mo dified by lymphotactin adenovirus, and fused with H22 cells by polyethylene glyc ol. RT-PCR and ELISA were employed to identify lymphotactin expression at mRNA a nd protein levels. The phenotypes and fusion efficiency were detected by FACS. T he stimulatory capacity of DC to T cells was detected by mixed leukocyte reactio n. The cytotoxicity activity against H22 cells was assayed by LDH method.RESULTS:
Lymphotactin effectively expressed by DCLptn/H22 hybrid oma. DCLptn/H22 cells induced potent T cell proliferation effect and generated s trong CTL reaction against allogenic H22 cells.CONCLUSION:
Lymphotactin genetic modification enhanced the in vitro immune activity of dendritoma.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
1986
Document type:
Article