GSH deletion increases the activity of p38MAPK in MNNG treatment / 中国病理生理杂志

ABSTRACT

AIM: To explore the effect of low concentration of N-methyl-N-nitro-N-nitroguanidine (MNNG) on p38MAPK, and the function of glutathione (GSH) on p38MAPK. METHODS: Western blotting was applied to detect the p38MAPK phosphorylation in MNNG treatment group and control group. To study the effect of GSH on MNNG-regulated p38MAPK activity, the intracellular GSH level was reduced by pretreatment of L-buthionine-S, R-sulfoximine (BSO). Assuming the absorbance of band in control group as 1.0, the relative P/T values of the treatment groups were calculated, with the “P” served as absorbance values of phospho-p38MAPK and the “T” as absorbance values of total p38MAPK. RESULTS: In BSO pretreated groups,the relative P/T in samples treated with MNNG for 2.5 h was 0.84, and became 2.19 with 3 h more incubation in the fresh medium. CONCLUSION: GSH deletion increases the activity of p38MAPK in MNNG treatment.