Effects of Recombinant Chimeric Toxin Dsg3EC_(1-2)PE40 on T and B Lymphocytes from Pemphigus Vulgaris / 中华皮肤科杂志
Chinese Journal of Dermatology
; (12)2003.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-525632
Responsible library:
WPRO
ABSTRACT
Objective To study the effects of recombinant chimeric toxin Dsg3EC_(1-2)PE40 on T and B cells from PV patients. Methods The recombinant protein Dsg3EC_(1-2)PE40 was expressed on BL21TrxB (DE3) cells, then identified and purified. ELISPOT assay was used to detect and quantitate autoantibody-producing B cells in different concentrations of recombinant chimeric toxin, and MTT assay and ~3H-TdR assay to observe the metabolism and proliferation of T cells from PV patients in vitro. Results The purity of expressed protein Dsg3EC_(1-2)PE40 was up to 80%. The number of anti-Dsg3 antibody-producing B cells in PBMC from PV patients decreased by 40% with treatment of Dsg3EC_(1-2)PE40, which was significantly lower (P
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Dermatology
Year:
2003
Document type:
Article