Determination of Acipimox in Human Serum by HPLC / 中国药房

ABSTRACT

OBJECTIVE: To establish HPLC method for the determination of acipimox in human serum. METHODS: The determination was performed on C18 column under room temperature, the mobile phase consisted of methanol -water(1585) with a flow rate of 0.8ml/ min and detection wavelength of 260nm, the quantitation was conducted by external reference method,the serum was subjected to protein sediment by methanol,the supernate fluid obtained thereafter was undergone sample injection. RESULTS: The linear range of acipimox was 1.0-16.0?g/ml (r = 0.9 987),the minimum detection concentration was 0.5?g/ ml; the average recovery of method was 96.05% . CONCLUSION: This method is simple, fast, sensitive and accurate, and which can be applied for blood concentration monitoring and pharmacokinetic study of acipimox.