Determination of Plasma Diclofenac Sodium by SPE-HPLC / 中国药房
China Pharmacy
; (12)2001.
Article
in Zh
| WPRIM
| ID: wpr-529025
Responsible library:
WPRO
ABSTRACT
OBJECTIVE: To establish an SPE(solid phase extraction)-HPLC method for the determination of diclofenac sodium in human plasma.METHODS: The separation of diclofenac sodium was performed on ODS-2 column with a detection wavelength of 280nm.The mobile phase was composed of phosphate buffer(pH6.5)-methanol(40∶ 60) at a flow rate of 1.0mL? min-1.RESULTS: The linear range of diclofenac sodium was 16.2~ 2 024.0ng? mL-1(r=0.998 9).The methodological recovery ranged between 90% to 110% and the extraction recovery was above 90%.Both intraday RSD and inter-day RSD were less than 5%.CONCLUSION: The method is simple,accurate and reliable,and suitable for the pharmacokinetic study of diclofenac sodium.
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
China Pharmacy
Year:
2001
Document type:
Article