Determination of Concentration of Irbesartan in Human Plasma by HPLC with Fluorescence Detection / 中国药房
China Pharmacy
; (12)2007.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-529635
Responsible library:
WPRO
ABSTRACT
OBJECTIVE:
To determine plasma concentration of irbesartan by HPLC with fluorescence detection.METHO-DSSamples were separated on Diamonsil C18 with mobile phase consisted of 0.02mol?L-1 KH2PO4-acetonitrile(50∶50) at a flow rate of 1.0mL?min-1.The fluorescence detector was set at an excitation wavelength of 250nm and an emission waveleng-th of 375nm.The column temperature was 40℃.RESULTS:
The linear range for irbesartan was from 1.0 to 1000ng?mL-1 (r=0.999 8).The mean relative recoveries of irbesartan at low,middle and high concentrations(2.0,50.0,and 500.00ng? mL-1) were 97.4%,106.1% and 101.6%,respectively.Both intra-day and inter-day RSD were below 10%.CONCLUSION:
The method is simple,rapid,accurate and specific,and it can be used for the plasma concentration determination and pharmacokinetic study of irbesartan.
Full text:
Available
Database:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
China Pharmacy
Year:
2007
Document type:
Article