Assaying of Tanshinone Ⅱ_A in Lianchuang Pills by HPLC / 中国药房

ABSTRACT

OBJECTIVE:To develop an HPLC method for the determination of Tanshinone ⅡA in Lianchuang pills. METHODS:The chromatographic separation was performed on Agilent Zorbax SB-C18 (250mm?4.6mm,5?m) column with column temperature at 25℃. The mobile phased consisted of methanol-water (75∶25) at a flow rate of 1.0mL?min-1.The detection wavelength was set at 270nm.RESULTS:The linear range of Tanshinone ⅡA was 0.020 2～0.202 0?g(r=0.999 9).The average recovery was 99.79%(RSD=1.01%,n=6). CONCLUSION:The method is simple, rapid and accurate, and it could be used for the quality control of Lianchuang pills.