Plerosis Effect of Sodium Magnesium Fructose Diphosphate on Hypoxia/ Reoxygenation Injury of My-ocardial Cells in Vitro / 中国药房

ABSTRACT

OBJECTIVE:To study the plerosis effect of sodium magnesium fructose diphosphate (FDPM) on hypoxia/reoxygenation (H/R) injury of myocardial cells and the possible mechanism. METHODS: The myocardial cells which had been primary cultured for 4 days were used to establish H/R injury model before being assigned to five groups H/R, H/R+FDPM (4.8, 2.4, 1.2 mg?mL-1). Then set up normal control with normal cells. The activities of lactate dehydrogenase(LDH), creatine phosphokinase (CPK) and the intracellular level of free calcium ([Ca2+]i) were detected after being treated with corresponding drugs, and the ultrastructures of myocardial cells were observed under transmission electron microscope. RESULTS: As compared with H/R group, FDPM groups significantly decreased the activities of LDH and CPK and the level of [Ca2+]i (P